If you apply for a job that requires multivariate stats, or whatever the topic of the second class was, then it might make a difference.

But even then, if you can show that you know the topic by picking it up during your research, the lack of this class won't make a difference.

For jobs that do not require multivariate stats, it will not make any difference.

(Slightly edited after seeing Stephan Kolassa's comment)

If you apply for a job that requires multivariate stats, or whatever the topic of the second class was, then it might make a difference.

But even then, if you can show that you know the topic by picking it up during your research, the lack of this class won't make too much difference.

For jobs that do not require multivariate stats, it will probably not make much difference.