Can I publish without affiliation ? Now I am not associated with any university and i want to publish one publication . Is there solution of this problem ?
You certainly can publish without an official affiliation. One of the advantages of the anonymous peer-reviewing is that reviewers should be concerned only about the content; Not the author information. Even if it's not an anonymous submission, your name and affiliation should have nothing to do with the quality of your submission.
I have seen affiliations like this:
Author Name Independent Researcher author email.
Some even with only the name and email address.