For some context, I have a Bachelors in Biology, and the pandemic forced me to pursue an online graduate program in another field, but I've wanted to get back into bio research for some time and gain some more experience. I've recently started working at a university microbiology research lab with a government grant. I've been working about a month, learning the protocols and shadowing the other associate present. Lately I've been struggling to get a satisfying yield out of purifying a plasmid from some E.coli cultures. I've communicated this throughout the process to both the other associate and the PI asking for potential ways to correct this, but recently, the PI brought up in conversation mentioning my other degree, as if to imply I wasn't qualified. He knew my background before hiring me and the way he phrased it made it seem like he was reconsidering that decision. I think this might foreshadow an upcoming termination if I fail to deliver results by the end of next week, and I want to ask how likely is that assessment.
I've done this protocol before with this lab with other plasmids, and I've managed to harvest a sufficient yield with those ones. I'm following the protocol as instructed and I've brought it up with both the other associate and the PI the difficulties I've been running into and asked what could be going on. The last we spoke, the PI has implied that I might be insufficiently trained or wasn't a good fit for benchwork, and to be honest I'm afraid that means I'm likely to be terminated soon. If that's the case, what can I expect from future job prospects, as I'm under the impression that it's somewhat of a career death sentence to have such an early termination in one's background. I really enjoy this job, I struggled to get a good job after receiving my Masters and I'm not entirely sure what to do with myself if I lose this one. The pandemic derailed my chances to do hands on research pre-baccaleaureate or as a grad-student, and I don't have think I have enough money, time, or references to try for another degree.