About a month ago I asked a professor for a letter of recommendation with 2 weeks notice, he agreed and wrote it. I found out about another opportunity but the deadline is tomorrow; I sent him an email Tuesday night asking if he could write me a letter of recommendation again but he hasn't replied. Could he possibly not be willing to write me another recommendation?
He doesn't have office hours until tomorrow at 2:00 which seems really late to be bringing it up to him. But, I don't want to bother him by sending him another email in case he's ignoring my request on purpose.