I broadly support most of the tips given in the previous answers, but I'd like to add some more general concerns which I feel that you should think of before you even draw the first line:
Take-home message: Think about this long and hard. If you had to describe what was exciting about your work in one single sentence, what would it be? What is the one thing you want people to remember about your work? About your presentation? Make sure you're 100% clear about this before you start writing your poster.
Clarity: Once you have your take-home message, make your entire poster subservient to it. Place it prominently in your title and make it crystal clear in your first paragraph, as EnergyNumbers and Ana suggest. Anything that's on your poster that does not contribute directly to the take-home message shouldn't be there.
Lead your readers/viewers: If your main argument requires a chain of explanations, display these prominently and mark them as such. Make the text flow follow the flow of your argument or reasoning. Place figures where they nail a point home, and nowhere else. Clip arts and colour can be cool, but don't use them if it will distract your readers from your take-home message or otherwise make their eyes wander.
Preparation: Try to think of the three questions people will ask you when you present your results, and try to answer them pre-emptively in the poster. Also try to be as prepared as possible to explain stuff while standing next to it, e.g. make sure it still has all the data you need to point to when making an argument.
This may all sound a bit reductionist, but remember that apart from the space restrictions, you're also dealing with time restrictions. People usually just browse posters while on their coffee break. Your job is to captivate them and make the most of that short break.
This might also all sound a bit too much like leading the viewer/reader like mindless cattle. Don't worry, though, they won't care. I've yet to hear anybody complain about an argument being presented too clearly. If anybody wants details beyond the take-home argument, you'll be standing there to give them, which is why you should be prepared and ready for questions.